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cytochrome c oxidase subunit 4  (Cell Signaling Technology Inc)


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    Cell Signaling Technology Inc cytochrome c oxidase subunit 4
    Voluntary exercise alters left ventricle gene and protein expression related to mitochondrial biogenesis and dynamics in high fat- and chow-fed mice. (A) Western blot analysis to assess purity of crude mitochondria isolation where left ventricle mitochondrial pellet expresses mitochondrial marker TOM70 and COXIV and supernatant expresses calnexin. Protein expression of (B) LCLAT1 and (C) MFN2 in left ventricle-isolated crude mitochondria and (D) LCLAT1, (E) PGC-1α, and (F) MFN2 in left ventricle tissue from male VET or sedentary mice fed an HFD or chow diet. Left ventricle mRNA expression of (G) OPA1 and (H) DRP1 in male VET or sedentary mice fed an HFD or chow diet. Values were calculated relative to 18S housekeeper. Calnexin and β-actin were used as internal controls of protein loading in left ventricle samples and COXIV as internal control of protein loading in crude mitochondria samples. Analysis was performed using two-way analysis of variance with Tukey’s post hoc test for multiple comparisons. Data are expressed as mean ± standard error of the mean. (B–C) n : 5–6 per group. (D–F) n = 7 per group. (G and H) n = 9 per group. CE = chow exercise; CS = chow sedentary; COXIV = cytochrome c oxidase subunit 4; DRP1 = dynamin-related protein 1; HE = high fat diet exercise; HFD = high fat diet; HS = high fat diet sedentary; LCLAT1 = lysocardiolipin acyltransferase 1; MFN2 = mitofusin-2; OPA1 = optic atrophy 1; PGC-1α = peroxisome proliferator-activated receptor gamma coactivator-1α; TOM70 = translocase of outer mitochondria membrane 70; VET = voluntary exercise training.
    Cytochrome C Oxidase Subunit 4, supplied by Cell Signaling Technology Inc, used in various techniques. Bioz Stars score: 96/100, based on 1262 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/product/cytochrome+c/COX+IV+Antibody/pmc12811436-71-70-77
    Average 96 stars, based on 1262 article reviews
    cytochrome c oxidase subunit 4 - by Bioz Stars, 2026-09
    96/100 stars

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    1) Product Images from "Influence of diet-induced obesity and voluntary exercise training on cardiac lipids and mitochondrial function in mice"

    Article Title: Influence of diet-induced obesity and voluntary exercise training on cardiac lipids and mitochondrial function in mice

    Journal: Journal of Sport and Health Science

    doi: 10.1016/j.jshs.2025.101095

    Voluntary exercise alters left ventricle gene and protein expression related to mitochondrial biogenesis and dynamics in high fat- and chow-fed mice. (A) Western blot analysis to assess purity of crude mitochondria isolation where left ventricle mitochondrial pellet expresses mitochondrial marker TOM70 and COXIV and supernatant expresses calnexin. Protein expression of (B) LCLAT1 and (C) MFN2 in left ventricle-isolated crude mitochondria and (D) LCLAT1, (E) PGC-1α, and (F) MFN2 in left ventricle tissue from male VET or sedentary mice fed an HFD or chow diet. Left ventricle mRNA expression of (G) OPA1 and (H) DRP1 in male VET or sedentary mice fed an HFD or chow diet. Values were calculated relative to 18S housekeeper. Calnexin and β-actin were used as internal controls of protein loading in left ventricle samples and COXIV as internal control of protein loading in crude mitochondria samples. Analysis was performed using two-way analysis of variance with Tukey’s post hoc test for multiple comparisons. Data are expressed as mean ± standard error of the mean. (B–C) n : 5–6 per group. (D–F) n = 7 per group. (G and H) n = 9 per group. CE = chow exercise; CS = chow sedentary; COXIV = cytochrome c oxidase subunit 4; DRP1 = dynamin-related protein 1; HE = high fat diet exercise; HFD = high fat diet; HS = high fat diet sedentary; LCLAT1 = lysocardiolipin acyltransferase 1; MFN2 = mitofusin-2; OPA1 = optic atrophy 1; PGC-1α = peroxisome proliferator-activated receptor gamma coactivator-1α; TOM70 = translocase of outer mitochondria membrane 70; VET = voluntary exercise training.
    Figure Legend Snippet: Voluntary exercise alters left ventricle gene and protein expression related to mitochondrial biogenesis and dynamics in high fat- and chow-fed mice. (A) Western blot analysis to assess purity of crude mitochondria isolation where left ventricle mitochondrial pellet expresses mitochondrial marker TOM70 and COXIV and supernatant expresses calnexin. Protein expression of (B) LCLAT1 and (C) MFN2 in left ventricle-isolated crude mitochondria and (D) LCLAT1, (E) PGC-1α, and (F) MFN2 in left ventricle tissue from male VET or sedentary mice fed an HFD or chow diet. Left ventricle mRNA expression of (G) OPA1 and (H) DRP1 in male VET or sedentary mice fed an HFD or chow diet. Values were calculated relative to 18S housekeeper. Calnexin and β-actin were used as internal controls of protein loading in left ventricle samples and COXIV as internal control of protein loading in crude mitochondria samples. Analysis was performed using two-way analysis of variance with Tukey’s post hoc test for multiple comparisons. Data are expressed as mean ± standard error of the mean. (B–C) n : 5–6 per group. (D–F) n = 7 per group. (G and H) n = 9 per group. CE = chow exercise; CS = chow sedentary; COXIV = cytochrome c oxidase subunit 4; DRP1 = dynamin-related protein 1; HE = high fat diet exercise; HFD = high fat diet; HS = high fat diet sedentary; LCLAT1 = lysocardiolipin acyltransferase 1; MFN2 = mitofusin-2; OPA1 = optic atrophy 1; PGC-1α = peroxisome proliferator-activated receptor gamma coactivator-1α; TOM70 = translocase of outer mitochondria membrane 70; VET = voluntary exercise training.

    Techniques Used: Expressing, Western Blot, Isolation, Marker, Control, Membrane

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    Article Snippet: Cutaneous Tcell lymphoma (CTCL) is a rare nonHodgkin lymphoma with limited durable treatment options and poor overall survival, underscoring the need for new therapeutic approaches.. Sanguinarine (SNG), a natural benzophenanthridine alkaloid, has demonstrated anticancer activity in several malignancies, but its potential role in CTCL remains unexplored.. We hypothesized that SNG induces oxidative stress and mitochondrial dysfunction, leading to the inhibition of the PI3K/AKT/GSK3 pathway and apoptosis in CTCL cells.

    Incubation:

    Article Title: Designing an apoptosis reporter by mutagenesis-based insertion of caspase-3 cleavage motif into green fluorescence protein
    Article Snippet: For Western blotting analysis, 30–60 μg protein lysates were separated in 8–12 % sodium dodecyl sulfate–polyacrylamide gels and transferred onto nitrocellulose membranes (#HAWP04700, Millipore, Billerica, MA, USA). .. The membranes were blocked with 5 % skim milk (#70166, Sigma-Aldrich) for 1 h and incubated overnight at 4 °C with primary antibodies against GFP (#Ab290, Abcam, Cambridge, MA, USA), caspase-3 (#9662, Cell Signaling Technology, Danvers, MA, USA), cleaved caspase-3 (#9664, CST), cytochrome C (#11940, CST), PARP (#9542, CST), and GAPDH (#LF-PA0212, Abfrontier, Seoul, Korea). .. Next, the membranes were washed five times with 10 mM Tris–HCl (pH 7.5) containing 150 mM NaCl and 0.2 % Tween-20 (TBST) and incubated with horseradish peroxidase-conjugated goat anti-rabbit immunoglobulin G (IgG) or anti-mouse IgG (1:5000, both from Sigma-Aldrich) for 1 h at room temperature.

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    Western Blot:

    Article Title: Novel nitric oxide-releasing triptolidenol derivatives suppress gastric cancer by targeting the folate one-carbon metabolism pathway and inducing ROS accumulation.
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    Bioprocessing:

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    Image Search Results


    Voluntary exercise alters left ventricle gene and protein expression related to mitochondrial biogenesis and dynamics in high fat- and chow-fed mice. (A) Western blot analysis to assess purity of crude mitochondria isolation where left ventricle mitochondrial pellet expresses mitochondrial marker TOM70 and COXIV and supernatant expresses calnexin. Protein expression of (B) LCLAT1 and (C) MFN2 in left ventricle-isolated crude mitochondria and (D) LCLAT1, (E) PGC-1α, and (F) MFN2 in left ventricle tissue from male VET or sedentary mice fed an HFD or chow diet. Left ventricle mRNA expression of (G) OPA1 and (H) DRP1 in male VET or sedentary mice fed an HFD or chow diet. Values were calculated relative to 18S housekeeper. Calnexin and β-actin were used as internal controls of protein loading in left ventricle samples and COXIV as internal control of protein loading in crude mitochondria samples. Analysis was performed using two-way analysis of variance with Tukey’s post hoc test for multiple comparisons. Data are expressed as mean ± standard error of the mean. (B–C) n : 5–6 per group. (D–F) n = 7 per group. (G and H) n = 9 per group. CE = chow exercise; CS = chow sedentary; COXIV = cytochrome c oxidase subunit 4; DRP1 = dynamin-related protein 1; HE = high fat diet exercise; HFD = high fat diet; HS = high fat diet sedentary; LCLAT1 = lysocardiolipin acyltransferase 1; MFN2 = mitofusin-2; OPA1 = optic atrophy 1; PGC-1α = peroxisome proliferator-activated receptor gamma coactivator-1α; TOM70 = translocase of outer mitochondria membrane 70; VET = voluntary exercise training.

    Journal: Journal of Sport and Health Science

    Article Title: Influence of diet-induced obesity and voluntary exercise training on cardiac lipids and mitochondrial function in mice

    doi: 10.1016/j.jshs.2025.101095

    Figure Lengend Snippet: Voluntary exercise alters left ventricle gene and protein expression related to mitochondrial biogenesis and dynamics in high fat- and chow-fed mice. (A) Western blot analysis to assess purity of crude mitochondria isolation where left ventricle mitochondrial pellet expresses mitochondrial marker TOM70 and COXIV and supernatant expresses calnexin. Protein expression of (B) LCLAT1 and (C) MFN2 in left ventricle-isolated crude mitochondria and (D) LCLAT1, (E) PGC-1α, and (F) MFN2 in left ventricle tissue from male VET or sedentary mice fed an HFD or chow diet. Left ventricle mRNA expression of (G) OPA1 and (H) DRP1 in male VET or sedentary mice fed an HFD or chow diet. Values were calculated relative to 18S housekeeper. Calnexin and β-actin were used as internal controls of protein loading in left ventricle samples and COXIV as internal control of protein loading in crude mitochondria samples. Analysis was performed using two-way analysis of variance with Tukey’s post hoc test for multiple comparisons. Data are expressed as mean ± standard error of the mean. (B–C) n : 5–6 per group. (D–F) n = 7 per group. (G and H) n = 9 per group. CE = chow exercise; CS = chow sedentary; COXIV = cytochrome c oxidase subunit 4; DRP1 = dynamin-related protein 1; HE = high fat diet exercise; HFD = high fat diet; HS = high fat diet sedentary; LCLAT1 = lysocardiolipin acyltransferase 1; MFN2 = mitofusin-2; OPA1 = optic atrophy 1; PGC-1α = peroxisome proliferator-activated receptor gamma coactivator-1α; TOM70 = translocase of outer mitochondria membrane 70; VET = voluntary exercise training.

    Article Snippet: The plate was centrifuged at 500 g for 5 min at 4°C, and 140 μL of MAS buffer containing 10 μg/mL cytochrome C (Thermo Fisher Scientific) was pipetted to each well.

    Techniques: Expressing, Western Blot, Isolation, Marker, Control, Membrane

    Voluntary exercise alters left ventricle gene and protein expression related to mitochondrial biogenesis and dynamics in high fat- and chow-fed mice. (A) Western blot analysis to assess purity of crude mitochondria isolation where left ventricle mitochondrial pellet expresses mitochondrial marker TOM70 and COXIV and supernatant expresses calnexin. Protein expression of (B) LCLAT1 and (C) MFN2 in left ventricle-isolated crude mitochondria and (D) LCLAT1, (E) PGC-1α, and (F) MFN2 in left ventricle tissue from male VET or sedentary mice fed an HFD or chow diet. Left ventricle mRNA expression of (G) OPA1 and (H) DRP1 in male VET or sedentary mice fed an HFD or chow diet. Values were calculated relative to 18S housekeeper. Calnexin and β-actin were used as internal controls of protein loading in left ventricle samples and COXIV as internal control of protein loading in crude mitochondria samples. Analysis was performed using two-way analysis of variance with Tukey’s post hoc test for multiple comparisons. Data are expressed as mean ± standard error of the mean. (B–C) n : 5–6 per group. (D–F) n = 7 per group. (G and H) n = 9 per group. CE = chow exercise; CS = chow sedentary; COXIV = cytochrome c oxidase subunit 4; DRP1 = dynamin-related protein 1; HE = high fat diet exercise; HFD = high fat diet; HS = high fat diet sedentary; LCLAT1 = lysocardiolipin acyltransferase 1; MFN2 = mitofusin-2; OPA1 = optic atrophy 1; PGC-1α = peroxisome proliferator-activated receptor gamma coactivator-1α; TOM70 = translocase of outer mitochondria membrane 70; VET = voluntary exercise training.

    Journal: Journal of Sport and Health Science

    Article Title: Influence of diet-induced obesity and voluntary exercise training on cardiac lipids and mitochondrial function in mice

    doi: 10.1016/j.jshs.2025.101095

    Figure Lengend Snippet: Voluntary exercise alters left ventricle gene and protein expression related to mitochondrial biogenesis and dynamics in high fat- and chow-fed mice. (A) Western blot analysis to assess purity of crude mitochondria isolation where left ventricle mitochondrial pellet expresses mitochondrial marker TOM70 and COXIV and supernatant expresses calnexin. Protein expression of (B) LCLAT1 and (C) MFN2 in left ventricle-isolated crude mitochondria and (D) LCLAT1, (E) PGC-1α, and (F) MFN2 in left ventricle tissue from male VET or sedentary mice fed an HFD or chow diet. Left ventricle mRNA expression of (G) OPA1 and (H) DRP1 in male VET or sedentary mice fed an HFD or chow diet. Values were calculated relative to 18S housekeeper. Calnexin and β-actin were used as internal controls of protein loading in left ventricle samples and COXIV as internal control of protein loading in crude mitochondria samples. Analysis was performed using two-way analysis of variance with Tukey’s post hoc test for multiple comparisons. Data are expressed as mean ± standard error of the mean. (B–C) n : 5–6 per group. (D–F) n = 7 per group. (G and H) n = 9 per group. CE = chow exercise; CS = chow sedentary; COXIV = cytochrome c oxidase subunit 4; DRP1 = dynamin-related protein 1; HE = high fat diet exercise; HFD = high fat diet; HS = high fat diet sedentary; LCLAT1 = lysocardiolipin acyltransferase 1; MFN2 = mitofusin-2; OPA1 = optic atrophy 1; PGC-1α = peroxisome proliferator-activated receptor gamma coactivator-1α; TOM70 = translocase of outer mitochondria membrane 70; VET = voluntary exercise training.

    Article Snippet: Immunoblotting was performed with antibodies against lysocardiolipin acyltransferase 1(LCLAT1, PA5-25627; Thermo Fisher Scientific), PGC-1α (ab191838; Abcam, Cambridge, UK), MFN2 (PA5-118059; Thermo Fisher Scientific), ANP (sc-18811; Santa Cruz, Dallas, TX, USA), tumor necrosis factor alpha (TNFα, 3707; Cell Signaling, Danvers, MA, USA), phosphorylated adenosine monophosphate-activated protein kinase (AMPK, 2531; Cell Signaling), AMPKα (2532; Cell Signaling), Perilipin 5 (PA1-46215; Thermo Fisher Scientific), translocase of outer mitochondria membrane 70 (TOM70, 65675; Cell Signaling), cytochrome c oxidase subunit 4 (COXIV, 4844; Cell Signaling), and calnexin (208880; Merck, Rahway, NJ, USA).

    Techniques: Expressing, Western Blot, Isolation, Marker, Control, Membrane

    SS-31 reduces radiation-induced mitochondrial-related apoptosis proteins in H9C2 cells and hiPSC-CMs . (A, C) Representative western blot images showing the expression of mitochondrial apoptosis-related proteins Bax and Bcl-2 in H9C2 cells (A) and hiPSC-CMs (C). (B, D) Quantification of corresponding protein expression levels, n = 3–10. Experimental groups include control, SS-31 treatment, 10 Gy radiation, and 10 Gy radiation plus SS-31 treatment, as indicated in the figure. (E) Representative western blot images of cytochrome c (Cyt c) distribution. Protein levels were analyzed in isolated mitochondrial and cytosolic fractions. (F) Quantitative analysis of Cyt c expression. Cyt c levels were normalized to VDAC1 for the mitochondrial fraction and β-actin for the cytosolic fraction. n = 3–4. All data are presented as mean ± SEM. * P < 0.05 vs control; ** P < 0.01 vs control; # P < 0.05 vs irradiation; ## P < 0.01 vs irradiation.

    Journal: Journal of Radiation Research

    Article Title: Mitochondrial-targeted SS-31 peptide attenuates radiation-induced cardiomyocyte senescence

    doi: 10.1093/jrr/rrag048

    Figure Lengend Snippet: SS-31 reduces radiation-induced mitochondrial-related apoptosis proteins in H9C2 cells and hiPSC-CMs . (A, C) Representative western blot images showing the expression of mitochondrial apoptosis-related proteins Bax and Bcl-2 in H9C2 cells (A) and hiPSC-CMs (C). (B, D) Quantification of corresponding protein expression levels, n = 3–10. Experimental groups include control, SS-31 treatment, 10 Gy radiation, and 10 Gy radiation plus SS-31 treatment, as indicated in the figure. (E) Representative western blot images of cytochrome c (Cyt c) distribution. Protein levels were analyzed in isolated mitochondrial and cytosolic fractions. (F) Quantitative analysis of Cyt c expression. Cyt c levels were normalized to VDAC1 for the mitochondrial fraction and β-actin for the cytosolic fraction. n = 3–4. All data are presented as mean ± SEM. * P < 0.05 vs control; ** P < 0.01 vs control; # P < 0.05 vs irradiation; ## P < 0.01 vs irradiation.

    Article Snippet: Membranes were blocked with 1% BSA or 1% non-fat milk in tris-buffered saline with Tween 20 (TBST) for 1 hour at room temperature, followed by overnight incubation at 4°C with the following primary antibodies: TNFα (MILLIPORE, AB1837P, 1:2000), IL-6 (Sigma, SAB5700632, 1:2000), p16 (Invitrogen, PA5–20379, 1:2000), p21 (Invitrogen, 14–6715-81, 1:2000), Bax (Invitrogen MA5–14003, 1:1000), Bcl-2 (Abcam, ab196495, 1:2000), Lamin B1 (CST, 13435, 1:2000), Caspase 7 (CST, 9492S, 1:2000), Caspase 8 (CST, 4790S, 1:2000), Caspase 9 (CST, 9508S, 1:1000), Cytochrome c (Medchemexpress, HY- P80102 , 1:3000), VDAC1 (Medchemexpress, HY- P80369 , 1:3000), OxPhos Rodent WB Antibody Cocktail (Invitrogen, 45–8099, 1:2000), or β-actin (Bio-techne, MAB8929, 1:5000).

    Techniques: Western Blot, Expressing, Control, Isolation, Irradiation